Small Molecule Enzymes in E. coli - Pathway Dataset

The pathways listed in the table below were used in our study. Clicking on the pathway name will bring up the pathway encoded in our (perl script parseable) 'npl' format. The EcoCyc pathway(s) used are listed. Clicking on these will take you to the appropriate EcoCyc web page(s).

The 'npl' files list reaction frames. In EcoCyc, these are the constituent parts of pathways. To identify the enzymes involved withtin a particular reaction frame, visit the following web page: http://ecocyc.org:1555/ECOLI/new-image?type=REACTION&object=REACTION-OF-INTEREST-RXN (replacing REACTION-OF-INTEREST-RXN with the relevant reaction identifier).

For example, to identify the enzymes involved in reaction frame GAPOXNPHOSPHN-RXN visit http://ecocyc.org:1555/ECOLI/new-image?type=REACTION&object=GAPOXNPHOSPHN-RXN.

Certain of `our' reaction frames were formed by merging two or more EcoCyc reaction frames. Such reaction frames always end with '-STURXN'. To obtain information about these frames, remove the '-STURXN', split the string at the '_' characters and append '-RXN' to each obtained fragment. For example, ACETALD-DEHYDROG_ALCOHOL-DEHYDROG-STURXN is composed of reaction frames ACETALD-DEHYDROG-RXN and ALCOHOL-DEHYDROG-RXN both of which can be looked up as indicated in the previous paragraph.

Pathways

Pathway Name Pathway Descriptions EcoCyc Pathway(s)
ARABCAT-PWY L-arabinose catabolism ARABCAT-PWY
DARABCAT-PWY D-arabinose catabolism DARABCAT-PWY
GLUTATHIONESYN-PWY glutathione biosynthesis GLUTATHIONESYN-PWY
GALACTITOLCAT-PWY galactitol catabolism GALACTITOLCAT-PWY
ELECTRON-TRANSPORT-2_ANA-ELECT-TRANS-PWY aerobic electron transfer and anaerobic electron transfer ELECTRON-TRANSPORT-2, ANA-ELECT-TRANS-PWY
PROPIONMET-PWY propionate metabolism, methylmalonyl pathway PROPIONMET-PWY
NADPHOS-DEPHOS-PWY NAD phosphorylation and dephosphorylation NADPHOS-DEPHOS-PWY
AMMASSIM-PWY ammonia assimilation cycle AMMASSIM-PWY
PROUT-PWY proline utilization PROUT-PWY
GLUCARGALACTSUPER-PWY D-glucarate and D-galactarate catabolism super-pathway GLUCARGALACTSUPER-PWY
LYXMET-PWY L-lyxose metabolism LYXMET-PWY
XYLCAT-PWY xylose catabolism XYLCAT-PWY
AERESPDON-PWY_ANARESPDON-PWY aerobic respiration, electron donors reaction list and anaerobic respiration, electron donors reaction list AERESPDON-PWY, ANARESPDON-PWY
ECASYN-PWY enterobacterial common antigen biosynthesis ECASYN-PWY
TRYPDEG-PWY tryptophan utilization TRYPDEG-PWY
PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis PYRIDOXSYN-PWY
PHOS-PWY phosphatidic acid and phospholipid biosynthesis PHOS-PWY
TREDEGLOW-PWY trehalose degradation, low osmolarity TREDEGLOW-PWY
ASPASN-PWY asparagine. ASPASN-PWY
GLUCOSE1PMETAB-PWY glucose 1-phosphate metabolism GLUCOSE1PMETAB-PWY
FASYN-INITIAL-PWY fatty acid biosynthesis, initial steps FASYN-INITIAL-PWY
AST-PWY arginine catabolism AST-PWY
PYRNUCYC-PWY_PYRIDNUCSYN-PWY pyridine nucleotide cycling and pyridine nucleotide synthesis PYRNUCYC-PWY, PYRIDNUCSYN-PWY
PENTOSE-P-PWY pentose phosphate pathway PENTOSE-P-PWY
DETOX1-PWY removal of superoxide radicals DETOX1-PWY
THIOREDOX-PWY thioredoxin pathway THIOREDOX-PWY
PYRUVOX-PWY pyruvate oxidation pathway PYRUVOX-PWY
FASYN-ELONG-PWY_UNSAT-FA-ELONG-PWY fatty acid elongation, saturated and fatty acid elongation, unsaturated FASYN-ELONG-PWY, UNSAT-FA-ELONG-PWY
FUC-RHAMCAT-PWY_METHGLYUT-PWY fucose and rhamnose catabolism and methylglyoxal metabolism FUC-RHAMCAT-PWY, METHGLYUT-PWY
RIBOSYN2-PWY riboflavin, FMN and FAD biosynthesis RIBOSYN2-PWY
CARNMET2-PWY carnitine metabolism CARNMET2-PWY
GLUT-REDOX-PWY glutathione-glutaredoxin redox reactions GLUT-REDOX-PWY
GLYCEROLMETAB-PWY glycerol metabolism GLYCEROLMETAB-PWY
BIOTIN-SYNTHESIS-PWY biotin biosynthesis BIOTIN-SYNTHESIS-PWY
ARG+POLYAMINE-SYN_ORNARGDEG-PWY arginine and polyamine biosynthesis and arginine and ornithine utilization superpathway ARG+POLYAMINE-SYN, ORNARGDEG-PWY
MENAQUINONESYN-PWY menaquinone biosynthesis MENAQUINONESYN-PWY
IDNCAT-PWY L-idonate catabolism IDNCAT-PWY
GALACTCAT-PWY galactonate catabolism GALACTCAT-PWY
SUGARCAT-PWY lactose, glucose and galactose degradation SUGARCAT-PWY
CYANCAT-PWY cyanate catabolism CYANCAT-PWY
PYRUVDEHYD-PWY pyruvate dehydrogenase PYRUVDEHYD-PWY
GLYCOCAT-PWY glycogen catabolism GLYCOCAT-PWY
PLPSAL-PWY pyridoxal 5'-phosphate salvage pathway PLPSAL-PWY
BRANCHED-CHAIN-AA+ALA-PWY_ALADEG-PWY _THREOCAT-PWY leu+val+ala+ile biosynthesis and L-alanine degradation and threonine catabolism BRANCHED-CHAIN-AA+ALA-PWY, ALADEG-PWY, THREOCAT-PWY
GALACT-GLUCUROCAT-PWY galacturonate and glucuronate catabolism GALACT-GLUCUROCAT-PWY
PPGPPMET-PWY ppGpp metabolism PPGPPMET-PWY
CARNMET-PWY carnitine metabolism, CoA-linked CARNMET-PWY
ACETATEUTIL-PWY acetate utilization ACETATEUTIL-PWY
BETSYN-PWY betaine biosynthesis BETSYN-PWY
ENTBACSYN-PWY enterobactin synthesis ENTBACSYN-PWY
COBALSYN-PWY cobalamin biosynthesis COBALSYN-PWY
HISTDEG-PWY histidine degradation HISTDEG-PWY
DEOXYRIBONUCMET-PWY deoxyribonucleotide metabolism DEOXYRIBONUCMET-PWY
HEXITOLDEGSUPER-PWY hexitol degradation super-pathway HEXITOLDEGSUPER-PWY
KDO-PEP-LIPASYN-PWY_GLCMANNANAUT-PWY KDO2-lipid A and peptidoglycan biosynthesis and N-acetylneuraminic acid KDO-PEP-LIPASYN-PWY, GLCMANNANAUT-PWY
LCYSDEG-PWY L-cysteine catabolism LCYSDEG-PWY
PYR-RIBONUCMET-PWY pyrimidine ribonucleotide/ribonucleoside metabolism PYR-RIBONUCMET-PWY
THISYN-PWY thiamin biosynthesis THISYN-PWY
ALL-CHORISMATE-PWY_1CMET2-PWY pathways of chorismate and formylTHF biosynthesis ALL-CHORISMATE-PWY, 1CMET2-PWY
DAPLYSINESYN-PWY lysine and diaminopimelate biosynthesis DAPLYSINESYN-PWY
SERDEG-PWY L-serine degradation SERDEG-PWY
PANTOSYN-PWY pantothenate and coenzyme A biosynthesis PANTOSYN-PWY
OANTIGEN-PWY O-antigen biosynthesis OANTIGEN-PWY
SER-GLYSYN-PWY serine and glycine biosynthesis SER-GLYSYN-PWY
PROSYN-PWY proline biosynthesis PROSYN-PWY
TRESYN-PWY trehalose biosynthesis TRESYN-PWY
HCAMHPDEG-PWY 3-phenylpropionate and 3-(3-hydroxyphenyl)propionate degradation HCAMHPDEG-PWY
NONMEVIPP-PWY isopentenyl diphosphate biosynthesis, mevalonate-independent NONMEVIPP-PWY
POLYISOPRENSYN-PWY polyisoprenoid biosynthesis POLYISOPRENSYN-PWY
GLYCOGENSYNTH-PWY glycogen biosynthesis GLYCOGENSYNTH-PWY
ACETOACETATE-DEG-PWY degradation of short-chain fatty acids ACETOACETATE-DEG-PWY
GLUTAMINDEG-PWY glutamine degradation I GLUTAMINDEG-PWY
GLYCOLYSIS/TCA/GLYOX-BYPASS_GLYCOLYSIS/E-D _ANARESP1-PWY glycolysis+TCA+glyoxylate bypass and glycolysis+Entner-Doudoroff and anaerobic respiration and fermentation and gluconeogenesis and glycol metabolism and degradation GLYCOLYSIS%2FTCA%2FGLYOX-BYPASS, GLYCOLYSIS%2FE-D, ANARESP1-PWY, FERMENTATION-PWY, GLUCONEO-PWY, GLYCOL-GLYOXDEG-PWY
SULFATE-CYS-PWY sulfate assimilation and cysteine biosynthesis SULFATE-CYS-PWY
MET-SAM-PWY_THRESYN-PWY methionine and methyl-donor-molecule biosynthesis and threonine biosynthesis MET-SAM-PWY, THRESYN-PWY
HEMESYN2-PWY biosynthesis of proto- and siroheme HEMESYN2-PWY
ANARESPACC-PWY anaerobic respiration, electron acceptors reaction list ANARESPACC-PWY
COLANSYN-PWY_MANGDPMET-PWY colanic acid biosynthesis and mannose and GDP-mannose metabolism COLANSYN-PWY, MANGDPMET-PWY
PRPP-PWY_NUCMET2-PWY his+purine+pyrimidine biosynthesis and nucleotide metabolism PRPP-PWY, NUCMET2-PWY
FAO-PWY fatty acid oxidation pathway FAO-PWY
DEOXYPYRNUCMET-PWY_RIB-DRIBMET-PWY deoxypyrimidine nucleotide/side metabolism and ribose and deoxyribose phosphate metabolism DEOXYPYRNUCMET-PWY, RIB-DRIBMET-PWY

The 'npl' Format

The'npl' format represents pathways as a series of connected threads (unbranched chains of reactions). The characters at the begining of the lines have the following meaning:

Character at
begining
of line
Meaning Character
at end
of line
>Start of file; followed by pathway IDN/A
\Pathway descriptionN/A
{Start of thread; contains thread number and first reaction frame in thread}
^Preceeding threads: the identifiers for the threads connected 'upstream' to the current one^
(Next reaction frame in thread: Line contains previous reaction frame identifier followed by next frame identifier)
$Subsequent threads: the identifiers for the threads connected 'downstream' to the current one$
#Comment lineN/A